Optimizing Synthetic RNAi: Perspectives from the Pharmaceutical and Biotech Industry

Table of Contents

Section 1. Analysis and Interpretation of Survey Results

• Executive Overview
• Study Objectives
• Customer Profile
  • Research Objectives
  • Experimental Design
  • Product Requirements
• Market Potential
  • Throughput and Market Growth
  • Future Trends
• Supplier Performance
  • Preferred Suppliers
  • Customer Satisfaction
  • Brand Share
  • Brand Health

Section 2. Study Methodology and Demographics

• Methodology
• Definition of Terms
• Demographics
• Questionnaire

Section 3. Presentation of Survey Data

• Experimental Design
  • Currently use synthetic siRNA
  • Primary research objective of RNAi experiments
  • Primary species studied in RNAi research
  • Cell types primarily used
  • Typical method of measuring the effectiveness of RNAiinduced gene silencing
  • Percent reduction in gene expression that is usually required
• siRNA Selection
  • Percentage of total RNAi experiments for which each type of synthetic siRNA is used
  • Preferred type of siRNA
  • Synthesis scale typically ordered for preferred type of siRNA
  • Order turn-around time normally required for preferred type of siRNA
  • Typical specifications for preferred type of siRNA
    • Strandedness
    • Quantity
    • Format
  • Level of purification usually ordered for preferred type of siRNA
  • Most common modification ordered for preferred type of siRNA
  • Preferred method for ordering pre-designed or validated siRNA
  • Primary way of designing custom siRNA
• Experimental Throughput
  • Typical number of unique synthetic siRNAs utilized per gene target
  • Average number of gene targets analyzed per month
  • Expected change in the number of gene targets analyzed
• Choosing the siRNA Supplier
  • Brands of synthetic siRNA currently used
  • Primary brand of synthetic siRNA
  • Percentage of RNAi experiments for which each brand of siRNA is used
  • Relative amount of money spent on each brand of siRNA
• Evaluating the Primary Supplier' s Performance
  • Satisfaction with specific product features of synthetic siRNA from primary supplier
    • Formats available
    • Modifications available
    • Purification options
    • Purity
    • Reduction of target gene expression
    • Specificity of gene suppression
    • Stability in cells
    • Time-to-results
    • Transfectability
  • Overall satisfaction with synthetic siRNA from primary supplier
  • Happiness with the performance of synthetic siRNA from primary supplier
  • Familiarity with other RNAi products from primary supplier
  • Level of acceptability of synthetic siRNA prices from primary supplier
  • Ease of obtaining synthetic siRNA from primary supplier
  • Helpfulness of customer service from primary supplier
  • Helpfulness of technical service from primary supplier
  • Likelihood of recommending synthetic RNAi products from primary supplier to a colleague
• Future Trends in Nucleic Acid-Based Gene Silencing
  • Identifying which molecules would be the most useful in advancing research goals in:
    • Gene function analysis
    • Genome-wide screening
    • Therapeutic applications
  • Improvement to siRNA that would most convince scientists to try a new supplier' s siRNA
• Demographics

Section 4. Appendices

• Insights and Perspectives
• Cross-Tabulations of Survey Data
• Other Recent Publications
• About BioInformatics
• Our Valued Clients