Report: Proteomics Survey Report 2003 TOC

I. METHODOLOGY AND DEMOGRAPHICS

II. PROTEOMICS GENERALLY

Q050. Proteomics Spending to Step Up in 2003
Q060. Majority Pursuing Multiple Proteomics Research Areas
Q067. Key to Future of Proteomics: Protein Antibody Chips, 2D/Mass Spec, and MDLC
Q070. Sample Prep the Key Bottleneck in Proteomics Research
Q080. System-Wide Proteomics Research Most Common

III. PROTEIN SEPARATION

Q090. Liquid Chromatography, Gels Remain the Dominant Separation Technologies
Q100. Separation Techniques Possess Clear Advantages & Disadvantages
Q110. Protein Separation Spending to Step Up in 2003
Q120. Adoption of Separation Technologies Likely in 2003
Q130. Differential Protein Expression Studies Most Common Reason for Protein Separation

IV. ULTRAFILTRATION

V. 1D and 2D GEL ELECTROPHORESIS

Q190. 1D Gel Electrophoresis Maintains Edge over 2D
Q200. SDS-PAGE is the Dominant 1D Gel Material
Q210. SDS-PAGE is the Dominant 2D Gel Material
Q220. Robotics Used in Minority of 1D Gel Processing
Q230. Robotics Used in Minority of 2D Gel Processing
Q240. Non-Fluorescent Dyes Dominate Gel Staining
Q250. Majority of Gels Used Solely for Image-Based Analysis
Q260. Spots per Gel Subject to Further Physical Analysis
Q270. 1D Gels Run Far More Frequently than 2D Gels
Q280. Substantial Increase in Use of 1D/2D Gels Expected
Q290. Increased Automation, Improved Resolution are Key Unmet Needs in Gel Electrophoresis
Q300. Respondents Typically Use Gel-Related Products from Three Vendors

V. 1D and 2D GEL ELECTROPHORESIS

Q310. Increased Automation and Improved Resolution/Sensitivity are Key Unmet Needs in Image Analysis
Q320. Gel Electrophoresis Spending to Step Up in 2003
Q322. Gel Consumables Spending to Step Up in 2003
Q324. Gel Equipment Spending to Step Up in 2003
Q326. Imaging-Related Product Spending to Step Up in 2003

VI. LIQUID CHROMATOGRAPHY

Q330. Reverse Phase and Affinity LC Most Commonly Used Methods
Q340. Large Increases Forecast for Use of Liquid Chromatography
Q350. Resolution Cited as Both Advantage and Disadvantage of Liquid Chromatography
Q360. Five Most Common LC Setups Account for Half of Proteomics-Related Chromatography
Q370. Six LC Setups to Account for Majority of Increased Use in Proteomics
Q410. Affinity/Reverse Phase Chromatography Most Likely to be Adopted
Q420. Respondents Applying MS to LC Separations Do So Frequently
Q430. LC Spending to Step Up in 2003
Q440. Bio-Rad and Applied Biosystems Leading Sources for LC Equipment and Supplies

VII. CAPILLARY ELECTROPHORESIS (CE)

VIII. MASS SPECTROMETRY

Q490. MALDI-TOF Dominant, Followed by ESI-QTOF
Q500. Liquid Chromatography and Gel Electrophoresis Most Common pre-MS Separation Techniques
Q510. MS Ionization Methods Compete on Speed, Ease of Sample Prep, and Ease of Interface with LC and MS
Q530. Sensitivity and Accuracy By Far the Most Important Attributes of MS Setup
Q550. MALDI-TOF, ESI-ION TRAP Lead List of Setups to be Acquired
Q570. Number of Spectra Generated per Month to Increase 300% in 2002-2003
Q590. Phosphorylation the Most Commonly Studied Post-Translational Modification
Q610. MALDI-TOF and MALDI-TOF-TOF Lead List of MS Setups to be Acquired
Q620. Respondents Typically Use MC-Related Products from Two Vendors
Q640. Premium Placed on Integrated MS Platforms Sourced from Single Vendor
Q650. Proteomics-related MS Study Spending to Step Up in 2003
Q660. Proteomics-related MS Equipment Spending to Step Up in 2003

IX. PROTEIN INFORMATICS

Q670. SWISS-2DPAGE the Most Commonly Used 2D Gel Database
Q690. Mascot the Most Commonly Used Peptide Mass Database
Q710. SWISS-PROT the Most Commonly Used Protein Sequence Database
Q740. One-Third of Respondents Plan to Substantially Increase Spending on 2D Gel Databases in 2002-2003
Q750. Protein Sequence Database Spending to Step Up in 2003

X. PROTEIN ARRAYS

Q760. Expression and Protein-Protein Interaction Studies are Most Common Uses of Protein Arrays
Q770. Use of Protein Arrays Dominated by "Home-Grown" Arrays
Q790. Protein Array Spending to Step Up in 2003

XI. PROTEIN QUANTIFICATION

Q830. Gels and Lowry/BCA/Bradford Assays Most Common Protein Quantification Methods

XII. PROTEIN LOCALIZATION

Q860. Immuno and Centrifugation/Fractionation Most Common Techniques in Protein Localization
Q870. Membrane-Bound Proteins Most Common Localization Targets

XIII. PROTEIN-PROTEIN INTERACTIONS

Q950. Yeast Two-Hybrid Dominant Technique in Studying Protein-Protein Interactions
Q960. Transient/Stable Transfection Dominant Measure in Mammalian Protein Interaction Studies

XIV APPENDIX