Next-Generation Sequencing: Solving the Genome

Table of Contents

Chapter 1 INTRODUCTION

• 1.1. Background
• 1.2. Scope and Nature of the Report

Chapter 2 HISTORY AND EVOLUTION OF SEQUENCING TECHNOLOGY

• 2.1. Frederick Sanger & Early DNA Sequencing
• 2.2. Leroy Hood Pioneers Semi-Automated Sequencing
• 2.3. The Human Genome Project and Celera Genomics: Race On
• 2.4. The $1,000 Genome & Next-Generation Systems

Chapter 3 SEQUENCING TECHNOLOGIES: NEXT-GENERATION AND BEYOND

• 3.1. Current Next-Generation Sequencers
  • Roche/454 Life Sciences
  • Illumina
  • Applied Biosystems
  • Helicos
• 3.2. Sequencers Currently in Development
  • Complete Genomics
  • BioNanomatrix
  • VisiGen
  • Pacific Biosciences
  • Dover Systems
  • Intelligent Bio-Systems
  • ZS Genetics
  • Reveo
  • LightSpeed Genomics
• 3.3. Nanopore-Based Systems
  • NABsys
  • Oxford Nanopore Technologies
• 3.4. Performance Comparisons
• 3.5. Bioinformatics
• 3.6. Sample Preparation
• 3.7. National Human Genome Research Institute (NHGRI) Funding
• 3.8. Intellectual Property Issues

Chapter 4 APPLICATIONS OF NEXT-GENERATION SEQUENCING TECHNOLOGIES

• 4.1. Whole-Genome Sequencing
  • The 1,000 Genomes Project
  • Exon Sequencing
  • Accuracy and Precision in Genetic Variation Studies
• 4.2. RNA Sequencing
• 4.3. Gene Regulation and Control Analysis
• 4.4. Disease Diagnosis and Prediction
  • Cancer Genomics
  • Personal Genomics
• 4.5. Metagenomics

Chapter 5 MARKET DYNAMICS

• 5.1. Deals
• 5.2. User Survey Results
  • Chief Application for Next-Generation DNA Sequencing
  • Main Organism for Next-Generation Sequencing
  • User Experience with Next-Generation Platform, Industry Segment
  • Next-Generation Platform Predicted to Dominate in 2 - 3 Years
  • Most Exciting Third-Generation Platform
  • Applications to Benefit Most from Next-Generation Sequencing
  • Research Areas to Benefit Most from Next-Generation Sequencing
  • Greatest Technical Concern about Next-Generation Sequencing Platforms
  • Greatest Computational/IT Concern
  • Adequate Cost/Benefit from Next-Generation Sequencing
  • Cost of Full Human Genome Sequence by 2010
  • Opinion on Platform Market Dominance
  • Future of Sanger Sequencing
  • Most Important Factors in Selecting a Next-Generation System
  • Opinion on Acquiring Sequence from a Service Provider
• 5.3. The Competitive Environment

Chapter 6 OBSERVATIONS AND CONCLUSIONS

Chapter 7 INTERVIEW TRANSCRIPTS

• 7.1. Clifford Reid, PhD, Chairman, President, & CEO, Complete Genomics
• 7.2. Patrice Milos, PhD, VP & Chief Scientific Officer, Helicos BioSciences
• 7.3. Steven Salzberg, PhD, Director, Center for Bioinformatics and Computational Biology, University of Maryland
• 7.4. Todd Smith, PhD, Chief Science Officer, Geospiza
• 7.5. Anonymous Academic Researcher Active in Next-Generation Sequencing
• 7.6. Stephen Naylor, PhD, CSO & Chairman, Predictive Physiology and Medicine (PPM)

References