G-Protein Coupled Receptors (GPCRs): Technical Overview, Emerging Technologies, Market Trends & Opportunities, 2nd Edition

Table of Contents

Chapter 1: Overview

• Scope of this Report: Technology and Market Trends in the GPCR Target Space 1--1
• Top Technical Trends in GPCR Research and Drug Discovery 1--2
• Unmet Needs in the GPCR Interrogation/Analysis Tools and Technology Space 1--3
• Cell-based Assays for GPCR Interrogation and Analysis: Pros and Cons 1--4
• Classification of GPCR Ligands 1--6
• Correlating Structure with Function for GPCRs 1--8
• GPCR Biology 1--9
• Half of All Pharmaceuticals Address GPCRs. Why Then is Drug Discovery Addressing GPCR Targets so Fraught with Uncertainty? 1--10
• GPCR Industry Challenges 1--13
• Contents of this Report 1--13

Chapter 2: Emerging Technologies and Areas of Focus in the GPCRs Space

• Scope 2--1
• Introduction to the Chapter 2--1
• Emerging Bioinformatics Approaches in the GPCRs Space: Predicting Ligands for Orphan GPCRs . 2--2
• Pairwise Sequence Searches 2--2
• Profile Methods 2--2
• Support Vector Machines 2--3
• Agglomerative Clustering Methods 2--4
• Motif-based Classifiers 2--4
• Alignment-independent Approaches to GPCR Classification 2--5
• Computational Approaches to Interrogate Aminergic GPCRs 2--7
• The Approach 2--8
• Emerging G-Protein Coupled Receptor Assay Technologies 2--9
• What can be Measured? 2--10
• Receptor Ligand Binding and GTP Binding Assays 2--10
• Second Messenger Assays 2--12
• Protein Interaction Assays 2--14
• Trafficking Assays 2--17
• Gene Reporter Assays 2--19
• Bioimpedance Assays 2--20
• Multiplexed GPCR Assays 2--20
• Screening Strategies 2--23
• Technical References in the GPCRs Space 2--24

Chapter 3: Qualitative and Quantitative Market Analysis of the GPCRs Space

• Introduction to the GPCR Marketplace 3--1
• Market Analysis of the GPCR Space Based on an End-User Survey 3--2
• Breakout of Survey Respondents 3--3
• Survey Responses of a Predominantly Quantitative Nature 3--3
• Survey Responses of a Predominantly Qualitative Nature 3--9
• Key issues and challenges affecting the whole GPCR marketplace as a whole 3--9
• Key technologies deployed for GPCR screening 3--13
• Quantitative Metrics of the GPCR Landscape 3--16
• The Intellectual Property Landscape in the GPCRs Space 3--18