Table of Contents
SECTION 1
CGH Highlights Copy Number Differences
- Eclectic Applications
- An Overview of the Methodology
- The Evolution of CGH
SECTION 2
The Scope of CGH Technology
- Methods
- Steps
- DNA Isolation and Preparation
- Amplification
- Labeling
- Hybridization on a Microarray
- Scanning and Analysis
- Limitations and Challenges
- Underlying Assumptions
- Copy Number Polymorphisms (CNPs)
- Distinguishing Loss of Heterozygosity
- Alternatives to CGH
SECTION 3
Applications of CGH
- Reproduction
- Assisted Reproduction Technologies
- Inherited Developmental Disorders
- Mental Retardation
- Multifactorial Disorders
- Cancer
- Evolution
SECTION 4
Expert Interviews
- Daniel Pinkel, PhD, University of California, San Francisco,
- Comprehensive Cancer Center
- Wendy Price, PhD, Invitrogen Corp.
- Peggy Eis, NimbleGen Systems Inc.
SECTION 5
Selected Company Profiles
- Affymetrix
- Agilent
- Invitrogen Corp.
- Molecular Devices Corp.
- NimbleGen Systems Inc.
- Signature Genomic Labs, LLC
APPENDIX
CHA Advances: Comparative Genomic Hybridization Survey, August 2006
References
Tables and Figures
- Figure 1. Outlook for Copy Number Variations in Clinical Testing
- Figure 2. Whole-Genome Analysis of a Human Tumor Cell Line
- Figure 3. Fine-Tiling Array CGH Analysis
- Figure 4. Steps in the CGH Process
- Figure 5. Selection of Products and Devices for CGH
- Figure 6. GeneChip Human Mapping 500K Array by Affymetrix
- Figure 7. SNP vs. CNP, Simplified
- Figure 8. Showing LOH Using BeadStudio
- Figure 9. Showing a Duplication Using Genome Viewer
- Figure 10. Types of Cancer Analyzed Using CGH
- Table 1. Selected Acquisitions
- Table 2. Selected Amplification Protocols Used in CGH Techniques
- Table 3. Selected Genomic Regions Interrogated with CGH
- Table 4. Selected Duplication/Deletion Disorders Detected with
CGH at Baylor
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