Gene Cloning and Protein Purification

Table of Contents

1 EXECUTIVE SUMMARY

• Scope and Methodology
  • Scope and Segmentation of the Research Service
  • Methodology
• Industry Scenario
  • Protein Purification and Gene Cloning
  • Scenario of Developments

2 TECHNOLOGY ADOPTION FACTOR ANALYSIS

• Technology Primer - Gene Cloning and Protein Purification
  • Gene Cloning
  • Gene Synthesis
  • Protein Purification
• Technology Drivers and Challenges
  • Drivers
  • Challenges
• Regulatory Issues in Gene Cloning and Analyst Insights
  • Gene Cloning to Therapy--Regulatory Issues
  • Analyst Insights

3 KEY INNOVATIONS IN PROTEIN PURIFICATION AND GENE CLONING

• Key Innovations in Gene Cloning
  • Real-Time PCR Enters the Next Level with Multiplexing
  • Hi-Fidelity DNA Polymerase Enhances the Speed of DNA Amplification
  • Advanced Baculovirus Expression Vector Enhances the Ease of Cloning
  • Fusion Cloning Eliminates Subcloning Procedures
  • Multiplexing Plasmid Minipreps Poised to Accelerate Cloning
  • Transposition Finds a Place in Gene Synthesis
  • Protein Extract Technology Enables Quick Isolation of Proteome
  • Gene Synthesis Technology Abolishes the Need for Natural DNA Templates
• Key Innovations in Protein Purification
  • A New Approach to Automated Affinity-Tagged Protein Purification
  • Nickel-Based Purification for 6xHistidine-Tagged Proteins
  • Novel Membrane Chromatography Approach for Purification
  • Pressure Cycling Technology Approach to Purify Protein from Lipid Rich Samples
  • Automation Platform for Protein DNA and RNA Purification
  • Kits and Columns--Necessity for Researchers
  • ProteomeLab Family Combines Partitioning and Fractionation
  • An Integrated System for Protein Purification

4 KEY PATENTS AND DATABASE OF KEY INDUSTRY PARTICIPANTS

• Patents
  • Patents in Gene Cloning
  • Patents in Protein Purification
• Database of Key Industry Participants
  • Industry Participants in Protein Purification
  • Industry Participants in Gene Cloning

5 DECISION SUPPORT DATABASES

• Database Tables
  • Percent Government R&D Investment in Biotechnology (2002-2012)
  • Global Pharmaceutical R&D Expenditure (2002-2012)
  • Global Percentage Pharmaceutical R&D Expenditure (2002-2012)